; SOURCE: /usr8/spider/docs/exa/atpk.spi ; AUTHOR: ArDean Leith Feb 2014 ; USAGE: spider spi/dat @atpk ; PURPOSE: Pick particles from micrograph using 'AT PK' ; ----------------- Input files -------------------------------------- [cemic] = 'sav_mic006_shrunk_ce' ; Decimated, enhanced micrograph ; ----------------- Output files ------------------------------------- [out] = 'atpk_doc' ; Alignment doc file ; ----------------- END BATCH HEADER --------------------------------- SYS echo " Filtering -----------------------------------------" ; echo FQ NP ; Fourier filtration [cemic] ; Histogram enhanced image (input) _1 ; Filtered incore image (output) 3 ; Gaussian low pass filter 25 ; Filter radius in pixels SYS echo " Testing 'AT PK' -----------------------------------" ; echo AT PK ; Find peaks _1 ; Image (input) 20 ; Search neighborhood 0.70 ; CC Peak threshold 70,70 ; Edge exclusion [out] ; Doc. file (output) SYS ; Display doc file cat [out].$DATEXT EN ! ----------------------- UNUSED BELOW HERE -------------------------,
.OPERATION | PS Z | ; Pick Slice - Z (xy plane) |
.INPUT FILE NAME | sav_crc_vol | ; File name |
.OUTPUT FILE NAME | psz_015 | ; Slice file name |
.SLICE NUMBER | 15 | ; Slice to be picked |