([doalign],[shrange],[step],[diam],[r1],[r2],[max-wait]) ;
; ; SOURCE: spider/docs/techs/recon/newprogs/apshgrp.spi ; New Nov 2004 ArDean Leith ; Rewritten Feb 2005 ArDean Leith ; Added alignment option & stack op Nov 2006 ArDean Leith ; SET MP location Feb 2008 ArDean Leith ; VAR name Feb 2010 ArDean Leith ; VAR names changed Dec 2010 ArDean Leith ; ; PURPOSE: Multi-reference alignment of an image series. ; Sample images are aligned with reference projections via ; shifts (translations) and rotations. ; FOR PROJECTS WITH MULTIPLE GROUPS- WITH OR WITHOUT PUBSUB. ; ;------------ Edit next 7 Input parameters ------------------------------ [doalign] = 1 ; If >0 apply alignment to images [shrange] = 8 ; Translation search range (must be divisible by step size) [step] = 2 ; Translation step size [diam] = 348 ; Diameter of object (Angstroms) for alignment search. ; (EDIT as needed!) Used to find last rotational alignment radius. [r1] = 5 ; First rotation alignment ring, only radii > this will be analyzed [r2] = 0 ; Last rotation ring (If zero, compute from object diameter) [max-wait] = 600 ; maximum headstart in seconds for copying stacks before next group starts ; ----------------- Input files -------------------------------------------- GLOBAL [params] = '../params' ; Parameter setting file GLOBAL [group_list] = 'sel_group' ; List of defocus groups (doc. file) GLOBAL [ref_images] = 'projs/prj_{***[grp]}@' ; Reference images GLOBAL [ref_images_angles] = 'refangles' ; Reference images angles doc. file GLOBAL [unaligned_images] = 'data{***[grp]}@' ; Unaligned particles images GLOBAL [group_particle_list] = 'sel_particles_***' ; Group particle list GLOBAL [mic2global_lut] = '../Particles/coords/mic2global****' ; Micrograph-to-particle lookup-table ; ----------------- Output files ------------------------------------------------ GLOBAL [temp_images] = '_8@' ; Local stacks (if fits in memory) ;GLOBAL [temp_images] = '/var/tmp/data{***[grp]}@' ; Local stacks (if doesn't fit in memory) GLOBAL [align_parameters] = 'align_01_***' ; Alignment parameters doc. file GLOBAL [align_params_all] = 'align_01_all' ; Combined alignment parameters GLOBAL [aligned_images] = 'dala01_{***[grp]}@' ; Rotated, shifted (aligned) particles GLOBAL [dummy_wait_file] = 'jnk_apshwait***' ; Dummy file created when finished copying GLOBAL [finished_flag] = 'flag-apsh-done_{***[grp]}' ; Dummy file created when finished ; ----------------- END BATCH HEADER ------------------------------------------- RE ;