Usage Example - Operation: ''TF ED''

Contrast Transfer Function - Estimate Defocus from image power spectrum

.OPERATION: TF ED [def],[cutoff] ;   Estimate Defocus
.INPUT FILE: pw_avg0006 ;   Power spectrum image (input)
.PIXEL SIZE [A] & SPHERICAL ABBERATION CS [MM]: 2.8, 2 ;   Pixel size, Spherical aberration
.WAVELENGTH LAMBDA [A]: 200 ;   Electron wavelength
.AMPLITUDE CONTRAST RATIO [0-1]: 0.1 ;   Ampl. contrast ratio
.DEFOCUS NOISE DOC FILE: tfed_noise ;   Noise doc file (output)


INPUT IMAGE
(Portion only)


INPUT POWER SPECTRUM IMAGE
pw_avg0006


OUTPUT NOISE DOC FILE
(Portion)
tfed _noise
RESULTS FILE
(Portion)
SPIDER Results file