LO I - Initialize files for Locating motif inside volume

PURPOSE

Create FFT of search volume, FFT of squared search volume, and FFT of rotated padded mask, as requested. These files are needed for: 'LO LSD' and 'LO'.

SEE ALSO

LO LSD	[Prepare LSD volume for Locating motif inside volume]
LO	[Locate motif inside a search volume]

USAGE

.OPERATION: LO I

.CREATE SEARCH FFT'S (S), MASK FFT (M), OR BOTH (B): B
[Select type of output files to be created. For a motif with a rotationally invariant mask, both types of output can be created initially before the motif is rotated. For a rotationally varying mask the mask fft must be recalculated at each orientation.]

.SEARCH VOLUME FILE: BIG000
[For efficiency this volume must be padded, if necessary, to even number of rows and slices before the operation can be used.]

If answer to initial question was 'S' or 'B' then following two queries appear:

.SEARCH VOLUME FFT OUTPUT FILE: search_fft
[Enter name for FFT of search volume.]

.SEARCH VOLUME SQ. FFT OUTPUT FILE: search_sq_fft
[Enter name for FFT of search volume squared.]

If answer to initial question was 'M' or 'B' then following two queries appear:

.ROTATED MOTIF MASK INPUT FILE: rot_mask
[Enter name of rotated motif mask.]

.PADDED MOTIF MASK FFT OUTPUT FILE: mask_fft
[Enter name for padded rotated motif mask FFT file.]

NOTES

1. The following procedures:
   sigs_settings.spi, sigs_pub.spi, and sigsloop.spi for an example of usage.

2. For futher info see:
   RAMOS (RApid MOtif Search) / Signature Search / Docking
   

3. References:
   B.K. Rath et al. (2003) Journal of Structural Biology 144, 95-103.
   Alan Roseman (2003) Ultramicroscopy 94, 225-236.
   

SUBROUTINES

MOTIF_INIT

CALLER

MOTIF1