SPIDER: TF COR (Transfer Function - CTF correction, image/volume)

TF COR - Transfer Function - CTF correction, image/volume

(10/19/15)

PURPOSE: CTF corrects an image/volume by Wiener filtering. Multiplies the FFT of the input image/volume by the CTF Fourier image. Then reverse FFT transforms this to create output image/volume. Similar to operation: 'TF CTS' without SNR adjustment. Further info on CTF related operations in SPIDER. Example.

SEE ALSO

TF CTS	[Transfer Function - CTF correction with SNR, image/volume]
TF SNR	[Transfer Function - CTF correction, using frequency dependent SNR]
TF CT	[Transfer Function - Generate a binary, phase flipping, complex, CTF correction image]
TF C	[Transfer Function - Generate a straight, complex, CTF correction image]
TF DDF	[Transfer Function - Determine Defocus & amplitude contrast]
TF DEV	[Transfer Function - Determine Envelope function]

USAGE

.OPERATION: TF COR

.IMAGE/VOLUME FILE: img001
[Enter template name of image/volume. Can be in Fourier format.]

.CTF FILE: ctf001
[Enter name of file containing complex CTF for this image/volume. ]

.OUTPUT FILE: img_ctfd
[Enter name for CTF corrected output file.]

NOTES

Input files can be either real or Fourier format. CTF files must be in Fourier format. All files must have the same dimensions. Output file is real.
Complex CTF files can be generated using 'TF C' or 'TF CT' for 2-D images or by using 'TF C3' or 'TF CT3' for 3-D volumes.
Theory is described in: J. Frank and P. Penczek (1995) On the correction of the contrast transfer function in biological electron microscopy. Optik 98:125-129.