INDEX OF SOME USEFUL SPIDER PROCEDURES

Task Uses operation
Align image series - using reference images. AP SH
Anistropic Diffusion - Selecting parameters CE AD
Anistropic Diffusion - Selecting parameters CE AD
Average an image series AS R
Back projection - conjugate gradient BP CG
Back projection - constrained iterative BP RP
Back projection - Fourier BP 3F, BP 32F
Back projection - weighted BP 3D
Filter reconstruction to a resolution limit FQ
FSC - Computation. RF 3
Mask image with a disk FD
Renumber image series VM
Remove duplicates from particle selection doc files DOC SORT
Rotate and shift images RT SQ
Rotate volume RT 3D
Sum an image series AD
Selection doc file - creation DOC CREATE
Segmentation - Test filter CE GNC, CE MED,
CE LAH, ED,
ER WA, CE RID, ...
Segmentation - Test thresholding TH F, FC,
FC E, CE
Motif searching (RAMOS) LO, LO I,
LO LSD
Threshold - remove outlying pixels. TH
Tomographic reconstruction - single tilt BP W2
Tomographic reconstruction - double tilt BP 3D

In addition the following techniques pages have links to numerous other SPIDER procedures.

Classification and Clustering
Electron Tomography
Particle Picking from Micrographs
Particle Verification using Classification
Projection Matching
Random Conical Tilt Reconstruction
Segmentation Using SPIDER
Segmenting RNA from protein
Signature Search / Motif Search / Docking

Source: procedure_index.html     Last update: 13 Feb. 2019     ArDean Leith